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PracticeQuiz and flashcards to consolidate what you studyStudy GuidesDisciplines, books and resources by courseInteractionsCheck drug, food, drink, disease and pregnancy interactionsMedicinesBrowse drugs by ATC classification, risk or nameMolecular TargetsCYPs, COX and transporters: what they are and how they explain interactions
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Molecular Targets

Enzymes, transporters and receptors that medicines modulate — what they are, what they do and how they explain interactions.

CYP450Cytochrome P450 3A4

CYP3A4

It is the most frequent target in interactions: drugs that inhibit it (e.g. ketoconazole, clarithromycin, grapefruit juice) raise substrate concentrations; drugs that induce it (e.g. rifampicin, carbamazepine, phenytoin) lower them and may cause loss of efficacy.

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CYP450Cytochrome P450 2D6

CYP2D6

CYP2D6 inhibition (e.g. fluoxetine, paroxetine) can double concentrations of substrates such as codeine, tramadol and metoprolol; in poor metabolisers, prodrugs activated by this enzyme (codeine → morphine) may have reduced effect.

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CYP450Cytochrome P450 2C9

CYP2C9

CYP2C9 inhibition (e.g. fluconazole, amiodarone, metronidazole, cotrimoxazole) is the central mechanism of interactions that raise INR with coumarins; induction (rifampicin) reduces the anticoagulant effect.

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CYP450Cytochrome P450 1A2

CYP1A2

CYP1A2 inhibition (e.g. ciprofloxacin + theophylline) increases the risk of theophylline toxicity (nausea, tachycardia, seizures); smokers who quit may accumulate substrates metabolised by this enzyme.

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CYP450Cytochrome P450 2C19

CYP2C19

CYP2C19 inhibition (e.g. omeprazole + clopidogrel) reduces formation of the active clopidogrel metabolite and may decrease antiplatelet protection; induction (rifampicin) accelerates substrate metabolism.

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CYP450Cytochrome P450 2C8

CYP2C8

CYP2C8 inhibition (e.g. gemfibrozil + repaglinide) raises substrate concentrations and the risk of hypoglycaemia; induction reduces the effect.

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CYP450Cytochrome P450 2B6

CYP2B6

CYP2B6 induction (e.g. rifampicin, phenobarbital) lowers efavirenz and bupropion concentrations; inhibition increases exposure.

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CYP450Cytochrome P450 2E1

CYP2E1

Chronic alcohol intake induces CYP2E1 and increases NAPQI formation from paracetamol, raising the risk of hepatotoxicity at therapeutic doses.

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COXCyclooxygenase 1

COX-1

COX-1 inhibition by NSAIDs and aspirin explains the gastrotoxic effects (ulcer, bleeding) and, with aspirin, the antiplatelet effect. It is the target of NSAID + anticoagulant/antiplatelet interactions.

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COXCyclooxygenase 2

COX-2

Selective COX-2 inhibitors spare COX-1 and have lower gastrointestinal risk, but retain cardiovascular (thromboembolic) and renal risk; interactions with anticoagulants remain relevant.

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TransportersP-glycoprotein (MDR1 / ABCB1)

P-gp (P-glycoprotein)

Drugs that inhibit P-gp (e.g. amiodarone, clarithromycin, ketoconazole, verapamil, diltiazem) increase absorption and concentrations of substrates such as digoxin, dabigatran and tacrolimus; inducers lower them.

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TransportersBreast cancer resistance protein (ABCG2)

BCRP

BCRP inhibition increases exposure to substrates such as rosuvastatin, methotrexate and sulfasalazine; combination with inhibitors (e.g. ciclosporin) may require dose reduction.

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TransportersOrganic anion transporting polypeptide 1B1 (SLCO1B1)

OATP1B1

OATP1B1 inhibition (e.g. ciclosporin, clarithromycin) reduces hepatic uptake of statins and raises systemic concentrations, with risk of myopathy and rhabdomyolysis.

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MAOMonoamine oxidase A

MAO-A

MAO-A inhibition raises synaptic monoamines; combination with SSRIs, tramadol, pethidine or dextromethorphan can cause serotonin syndrome, and tyramine-rich food can cause a hypertensive crisis.

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MAOMonoamine oxidase B

MAO-B

Selective MAO-B inhibition at therapeutic doses has lower tyramine interaction risk, but combination with serotonergic drugs (SSRIs, pethidine) retains serotonin syndrome risk.

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EnzymesVitamin K epoxide reductase

VKORC1

Warfarin and acenocoumarol inhibit VKORC1, reducing synthesis of vitamin K-dependent factors; dietary vitamin K intake antagonises this effect and shifts the INR.

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EnzymesGlucose-6-phosphate dehydrogenase

G6PD

Oxidant drugs can cause acute haemolysis in G6PD-deficient patients: primaquine, sulfonamides (cotrimoxazole), nitrofurantoin, dapsone, high-dose aspirin.

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EnzymesThiopurine S-methyltransferase

TPMT

Drugs that inhibit TPMT (e.g. allopurinol) increase azathioprine toxicity; allopurinol + azathioprine is the classic interaction and requires azathioprine dose reduction.

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EnzymesPhosphodiesterase 5

PDE5

Combining PDE5 inhibitors with nitrates (nitroglycerin, isosorbide) causes severe, potentially fatal hypotension, by potentiating nitric oxide; they also potentiate hypotension with alpha-blockers and riociguat.

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Enzymes3-hydroxy-3-methylglutaryl-CoA reductase

HMG-CoA reductase

Statins (simvastatin, atorvastatin) are metabolised by CYP3A4 and transported by OATP1B1; inhibition by ketoconazole, clarithromycin, ciclosporin or amiodarone increases the risk of myopathy and rhabdomyolysis.

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